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divIB [2020-08-07 10:06:43]
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divIB [2020-08-07 10:06:43]

cell-division initiation protein (septum formation)
locus
BSU_15240
pI
9.24
mw
30.02 kDa
protein length
263 aa Sequence Blast
gene length
792 bp Sequence Blast
function
septum formation
product
cell-division protein, member of the [SW|divisome]
essential
yes
synonyms
dds,tms-12
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,593,704 → 1,594,495

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Protein family

  • FtsQ/DivIB family (single member, according to UniProt)

    • [SW|Domains]

  • POTRA domain (aa 54-123) (according to UniProt)

    • Structure

  • [PDB|2ALJ] (major extracytoplasmic domain, Geobacillus stearothermophilus)

    • [SW|Localization]

  • cell membrane [Pubmed|9426141]

    • Expression and Regulation

    Operons

    genes
    [gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
    description
    [Pubmed|18179421]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, (promoter within [protein|D52FDB5015B10E9B26D7FED5708457890C6EA43D|MurG]) [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8106328], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|8320223], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

    • regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|1391053]
    • view in new tab

    genes
    [gene|D48B7591467C493D2214D37AEA83643FE8C27BC6|murE]-[gene|4D3D4429E1F387BACB0D5A1084AA8DF179A16685|mraY]-[gene|0A9FF5A66F73464A780914A1424979CE753B3D9C|murD]-[gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
    description
    [Pubmed|1391053]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|8320223], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

    • regulation

  • expressed during vegatative growth [Pubmed|1391053]
    • view in new tab

    genes
    [gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
    description
    [Pubmed|1391053]

    regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|1391053]
    • view in new tab

    genes
    [gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
    description
    [Pubmed|8106328]
    view in new tab

    genes
    [gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
    description
    [Pubmed|18179421]

    regulation

  • expressed during vegatative growth, then switched off, and again expressed during [SW|sporulation ]in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|1391053]
    • view in new tab

    Biological materials

    FLAG-tag construct

  • GP2005 ''divIB-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

    • References

  • 8459777,10852898,19429628,1391053,21219466,8320223,8106328,18179421,15659156,16936019,10217758,10792716,9720875,16936026,9426141,9044263,29443967